Slc26a5^tm1Jnz
Targeted Allele Detail

Summary

• Symbol: Slc26a5^tm1Jnz
• Name: solute carrier family 26, member 5; targeted mutation 1, Jian Zuo
• MGI ID: MGI:2387795
• Synonyms: prestin-KO
• Gene: Slc26a5 Location: Chr5:22015653-22070602 bp, - strand Genetic Position: Chr5, 9.97 cM, cytoband A3
• Alliance: Slc26a5^tm1Jnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79029
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 through 7 were replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region encoded 245 amino acids encompassing the start codon, a conserved STAT motif, positively charged residues involved in intracellular anion binding, and the first 5 of the the 12 transmembrane domains. Neither transcript nor protein was detected in homozygous mice by RT-PCR and Western blot analyses. (J:79029)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc26a5 Mutation: 54 strains or lines available

References

• Original: J:79029 Liberman MC, et al., Prestin is required for electromotility of the outer hair cell and for the cochlear amplifier. Nature. 2002 Sep 19;419(6904):300-4
• All: 17 reference(s)