Cdkn1b^tm1Jro
Targeted Allele Detail

Summary

• Symbol: Cdkn1b^tm1Jro
• Name: cyclin dependent kinase inhibitor 1B; targeted mutation 1, James M Roberts
• MGI ID: MGI:2387694
• Synonyms: p27^T187A
• Gene: Cdkn1b Location: Chr6:134897364-134902476 bp, + strand Genetic Position: Chr6, 65.77 cM
• Alliance: Cdkn1b^tm1Jro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71780
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The gene was replaced with a construct composed of a loxP-flanked stop box element within the promoter region and a gene carrying a threonine to alanine substitution at position 187 (T187A). The lox-STOP-lox element was removed by breeding homozygous animals to a line expressing cre recombinase constitutively in the germ line thereby allowing expression of the mutant allele in the next generation. (J:71780)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cdkn1b^tm1Jro

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkn1b Mutation: 25 strains or lines available

References

• Original: J:71780 Malek NP, et al., A mouse knock-in model exposes sequential proteolytic pathways that regulate p27Kip1 in G1 and S phase. Nature. 2001 Sep 20;413(6853):323-7
• All: 9 reference(s)