Evpl^tm1Fmw
Targeted Allele Detail

Summary

• Symbol: Evpl^tm1Fmw
• Name: envoplakin; targeted mutation 1, Fiona M Watt
• MGI ID: MGI:2387422
• Gene: Evpl Location: Chr11:116111385-116128903 bp, - strand Genetic Position: Chr11, 81.02 cM, cytoband E1
• Alliance: Evpl^tm1Fmw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71626
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by deleting and replacing exons 1-8 with an IRES-lacZ-neo cassette via homologous recombination. The insertion introduces stop codons in all 3 reading frames. Absence of gene expression in homozygous mutant animals was verified by RT-PCR analysis of skin samples using primers to both the 5' end and the 3' end of the gene. (J:71626)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Evpl Mutation: 68 strains or lines available

References

• Original: J:71626 Maatta A, et al., Gene targeting of envoplakin, a cytoskeletal linker protein and precursor of the epidermal cornified envelope. Mol Cell Biol. 2001 Oct;21(20):7047-53
• All: 5 reference(s)