Timp3^tm1Rkho
Targeted Allele Detail

Summary

• Symbol: Timp3^tm1Rkho
• Name: tissue inhibitor of metalloproteinase 3; targeted mutation 1, Rama Khokha
• MGI ID: MGI:2387409
• Synonyms: timp-3^-, Timp-3-null
• Gene: Timp3 Location: Chr10:86136276-86185369 bp, + strand Genetic Position: Chr10, 42.83 cM, cytoband C1-D1
• Alliance: Timp3^tm1Rkho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71609
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of part of exon 1 (just upstream of the translation initiation codon) to part of exon 2 (after the conserved VIRAK codons) with a neomycin resistance cassette in the reverse orientation. Northern blot of lung, kidney, and heart RNA and RT-PCR analysis did not detect gene expression in homozygous mutants. Reverse zymographic analysis of kidney extracts did not detect protein activity in homozygous animals. (J:71609)

Disease models

Expression

In Mice Carrying this Mutation:	46 assay results
In Structures Affected by this Mutation:	16 anatomical structures

Tumor Data

List all tumor models in MMHCdb carrying Timp3^tm1Rkho

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Timp3 Mutation: 12 strains or lines available

References

• Original: J:71609 Leco KJ, et al., Spontaneous air space enlargement in the lungs of mice lacking tissue inhibitor of metalloproteinases-3 (TIMP-3). J Clin Invest. 2001 Sep;108(6):817-29
• All: 48 reference(s)