Runx1^tm3Dow
Targeted Allele Detail

Summary

• Symbol: Runx1^tm3Dow
• Name: runt related transcription factor 1; targeted mutation 3, James R Downing
• MGI ID: MGI:2387301
• Synonyms: AML1/ETO, AML1-ETO knockin, AML1-ETO-stop, Aml^LSL-A/E, Runx1^{tm3(RUNX1-CBFA2T1)Dow}
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm3Dow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77129
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A floxed stop cassette containing a strong polyA signal was inserted into intron 3, upstream of the murine/human hybrid sequence engineered from human t(8;21) cDNA and used to generate Runx1^tm2Dow. While trace levels of an alternatively spliced transcript including the downstream region were detected, no such protein was identified in mutant mice by Western blot analysis. Excision of the floxed polyA signal and the resultant expression of the inserted human sequence was achieved when mice carrying this allele were bred with transgenic mice expressing cre recombinase. (J:77129)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 35 strains or lines available

References

• Original: J:77129 Higuchi M, et al., Expression of a conditional AML1-ETO oncogene bypasses embryonic lethality and establishes a murine model of human t(8;21) acute myeloid leukemia. Cancer Cell. 2002 Feb;1(1):63-74
• All: 7 reference(s)