Skil^tm1Sis
Targeted Allele Detail

Summary

• Symbol: Skil^tm1Sis
• Name: SKI-like; targeted mutation 1, Shunsuke Ishii
• MGI ID: MGI:2387096
• Synonyms: sno^-/-
• Gene: Skil Location: Chr3:31149259-31176741 bp, + strand Genetic Position: Chr3, 15.12 cM
• Alliance: Skil^tm1Sis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62174
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: The exon encoding amino acids 1 through 362 and common to all known isoforms produced from the endogenous locus was replaced by a neomycin selection cassette inserted via homologous recombination. Western blot analyses using antibodies for both amino and carboxy terminals showed protein levels in heterozygous mutant mice to be approximately half of the wild-type levels. (J:62174)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Skil Mutation: 38 strains or lines available

References

• Original: J:62174 Shinagawa T, et al., The sno gene, which encodes a component of the histone deacetylase complex, acts as a tumor suppressor in mice. EMBO J. 2000 May 15;19(10):2280-91
• All: 1 reference(s)