Nova1^tm1Rbd
Targeted Allele Detail

Summary

• Symbol: Nova1^tm1Rbd
• Name: NOVA alternative splicing regulator 1; targeted mutation 1, Robert B Darnell
• MGI ID: MGI:2386947
• Gene: Nova1 Location: Chr12:46744678-46866143 bp, - strand Genetic Position: Chr12, 21.46 cM
• Alliance: Nova1^tm1Rbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60771
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 and 2 were replaced with an IRES-lacZ-floxed-neo cassette via homologous recombination. The floxed neo cassette was removed in correctly targeted ES cells by transient expression of cre recombinase. Absence of gene expression in homozygous mutant animals was demonstrated by Western blot of hindbrain and forebrain extracts using anti-POMA antisera which recognizes all species of nova proteins. The Nova1-specific band was not observed in samples from homozygous mutants. (J:60771)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nova1 Mutation: 21 strains or lines available

References

• Original: J:60771 Jensen KB, et al., Nova-1 regulates neuron-specific alternative splicing and is essential for neuronal viability. Neuron. 2000 Feb;25(2):359-71
• All: 10 reference(s)