H1f1^tm1Drab
Targeted Allele Detail

Summary

• Symbol: H1f1^tm1Drab
• Name: H1.1 linker histone, cluster member; targeted mutation 1, Birgit Drabent
• MGI ID: MGI:2386881
• Synonyms: H1.1-deficient
• Gene: H1f1 Location: Chr13:23947649-23948389 bp, + strand Genetic Position: Chr13, 9.91 cM, cytoband A3.2
• Alliance: H1f1^tm1Drab page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60631
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region of the gene (from the translation initiation codon to 463 bp of the 3' flanking region) was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was demonstrated by RNase protection assay and Western blot analysis. Furthermore, in situ hybridization and immunohistochemical analysis of testes from homozygous mutant animals confirmed the absence of RNA and protein products. (J:60631)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H1f1 Mutation: 6 strains or lines available

References

• Original: J:60631 Rabini S, et al., Spermatogenesis in mice is not affected by histone H1.1 deficiency. Exp Cell Res. 2000 Feb 25;255(1):114-24
• All: 4 reference(s)