Fap^tm1Schn
Targeted Allele Detail

Summary

• Symbol: Fap^tm1Schn
• Name: fibroblast activation protein; targeted mutation 1, Andreas Schnapp
• MGI ID: MGI:2386791
• Synonyms: Fap^-
• Gene: Fap Location: Chr2:62331280-62404365 bp, - strand Genetic Position: Chr2, 35.85 cM
• Alliance: Fap^tm1Schn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59998
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4, intron 4, and part of exon 5 were replaced with a PGK-neo cassette via homologous recombination. Mutant gene transcripts were detected in homozygous mutant embryos by RNase protection assay and RT-PCR analysis. Sequencing of the RT-PCR product revealed the mutant transcript was spliced from exon 3 to exon 6 resulting in a frameshift and nonsense mutation in exon 7. Absence of protein product in homozygous mutants was confirmed by immunohistochemical analysis of E13.5 embryo sections and FAP-specific activity assays. (J:59998)

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fap Mutation: 54 strains or lines available

References

• Original: J:59998 Niedermeyer J, et al., Targeted disruption of mouse fibroblast activation protein. Mol Cell Biol. 2000 Feb;20(3):1089-94
• All: 7 reference(s)