Cnmd^tm1Ref
Targeted Allele Detail

Summary

• Symbol: Cnmd^tm1Ref
• Name: chondromodulin; targeted mutation 1, Reinhard Fassler
• MGI ID: MGI:2386777
• Gene: Cnmd Location: Chr14:79875130-79899610 bp, - strand Genetic Position: Chr14, 42.74 cM, cytoband D3
• Alliance: Cnmd^tm1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78596
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing an IRES-lacZ gene followed by a floxed neo gene was inserted into the first coding exon of the endogenous locus via homologous recombination. Transcript was undetected in homozygous mutant mice by Northern blot analysis of limb cartilage and RT-PCR analysis of cartilage, thymus, and whole eye tissue. Immunohistochemical analysis of postnatal tibial growth plates confirmed the absence of protein. (J:78596)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnmd Mutation: 24 strains or lines available

References

• Original: J:78596 Brandau O, et al., Chondromodulin I is dispensable during enchondral ossification and eye development. Mol Cell Biol. 2002 Sep;22(18):6627-35
• All: 3 reference(s)