Pdha1^tm1Ptl
Targeted Allele Detail

Summary

• Symbol: Pdha1^tm1Ptl
• Name: pyruvate dehydrogenase E1 alpha 1; targeted mutation 1, Mulchand S Patel
• MGI ID: MGI:2386703
• Synonyms: Pdha1^flox8
• Gene: Pdha1 Location: ChrX:158905205-158921409 bp, - strand Genetic Position: ChrX, 73.95 cM, cytoband F3-F4
• Alliance: Pdha1^tm1Ptl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74192
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 8 was left flanked by loxP sites after a downstream floxed tk-neo cassette was excised by the in vitro expression of cre recombinase. Western blot analysis of homozygous mutant ES cells showed normal levels of both isoforms produced by the endogenous allele. Mice carrying this allele were bred to transgenic mice expressing cre in the germline to generate Pdha1^tm1.1Ptl. (J:74192)

Expression

In Structures Affected by this Mutation:

21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdha1 Mutation: 23 strains or lines available

References

• Original: J:74192 Johnson MT, et al., Inactivation of the murine pyruvate dehydrogenase (Pdha1) gene and its effect on early embryonic development. Mol Genet Metab. 2001 Nov;74(3):293-302
• All: 24 reference(s)