Slc18a2^tm1Mca
Targeted Allele Detail

Summary

• Symbol: Slc18a2^tm1Mca
• Name: solute carrier family 18 (vesicular monoamine), member 2; targeted mutation 1, Marc G Caron
• MGI ID: MGI:2386330
• Synonyms: VMAT2^-
• Gene: Slc18a2 Location: Chr19:59249328-59284444 bp, + strand Genetic Position: Chr19, 54.64 cM
• Alliance: Slc18a2^tm1Mca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45184
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The sequences encoding transmembrane domains 3 and 4 were deleted and replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis and Western blot of whole brain RNA and synatpic vesicles from newborn animals. Binding assays using proteins prepared from synaptic vesicles showed no binding activity in the homozygous mutants. (J:45184)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc18a2 Mutation: 29 strains or lines available

References

• Original: J:45184 Wang YM, et al., Knockout of the vesicular monoamine transporter 2 gene results in neonatal death and supersensitivity to cocaine and amphetamine. Neuron. 1997 Dec;19(6):1285-96
• All: 9 reference(s)