Rad51d^tm1Jcs
Targeted Allele Detail

Summary

• Symbol: Rad51d^tm1Jcs
• Name: RAD51 paralog D; targeted mutation 1, John C Schimenti
• MGI ID: MGI:2386281
• Synonyms: Rad51^-, Rad51^tm1Jcs
• Gene: Rad51d Location: Chr11:82767260-82781440 bp, - strand Genetic Position: Chr11, 50.3 cM
• Alliance: Rad51d^tm1Jcs page

Delayed development and turning, posterior truncation, brain vesicle and limb bud defects in Rad51d^tm1Jcs/Rad51d^tm1Jcs embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61460
• Parent Cell Line: v6.4 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 4-6 with a neomycin resistance cassette via homologous recombination. The targeted mutation results in the loss of a conserved DNA-binding motif called the Walker box, and also causes a frameshift. Homozygous mutant embryos were detected by PCR genotype analysis up to day E10.5. The targeted allele was confirmed by RT-PCR analysis of E8.5 embryos using primers flanking the deleted region. No RT-PCR products were detected. (J:61460)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad51d Mutation: 24 strains or lines available

References

• Original: J:61460 Pittman DL, et al., Midgestation lethality in mice deficient for the RecA-related gene, Rad51d/Rad51l3. Genesis. 2000 Mar;26(3):167-73
• All: 2 reference(s)