Igfbp2^tm1Jep
Targeted Allele Detail

Summary

• Symbol: Igfbp2^tm1Jep
• Name: insulin-like growth factor binding protein 2; targeted mutation 1, John E Pintar
• MGI ID: MGI:2386117
• Gene: Igfbp2 Location: Chr1:72863662-72891633 bp, + strand Genetic Position: Chr1, 36.94 cM
• Alliance: Igfbp2^tm1Jep page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64338
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 and 0.6 kb of downstream intronic sequences containing the exon/intron boundary was replaced with a neomycin resistance gene via homologous recombination. Absence of mRNA and protein expression in homozygous mutant animals was confirmed by Northern blot analysis of brain and liver tissue using a cDNA probe to rat Igfbp2 and by Western blot analysis of serum proteins. (J:64338)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igfbp2 Mutation: 22 strains or lines available

References

• Original: J:64338 Wood TL, et al., Selective alterations in organ sizes in mice with a targeted disruption of the insulin-like growth factor binding protein-2 gene. Mol Endocrinol. 2000 Sep;14(9):1472-82
• All: 11 reference(s)