Nfe2l1^tm1Ywk
Targeted Allele Detail

Summary

• Symbol: Nfe2l1^tm1Ywk
• Name: nuclear factor, erythroid derived 2,-like 1; targeted mutation 1, Yuet Wai Kan
• MGI ID: MGI:2385929
• Synonyms: Nrf-1 -, Nrf1r^PGK-neo
• Gene: Nfe2l1 Location: Chr11:96708240-96720794 bp, - strand Genetic Position: Chr11, 60.12 cM, cytoband D-E
• Alliance: Nfe2l1^tm1Ywk page

Abnormal development of Nfe2l1^tm1Ywk/Nfe2l1^tm1Ywk Nfe2l2^tm1Ywk/Nfe2l2^tm1Ywk embryos with increased apoptosis

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46990
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into the terminal exon which encodes the DNA binding and leucine zipper domains. Northern blot and RT-PCR analysis demonstrated that a mutant 7 kb transcript was expressed from this allele, but immunoblot analysis confirmed that no detectable protein was encoded by this allele. (J:46990)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfe2l1 Mutation: 39 strains or lines available

References

• Original: J:46990 Chan JY, et al., Targeted disruption of the ubiquitous CNC-bZIP transcription factor, Nrf-1, results in anemia and embryonic lethality in mice. EMBO J. 1998 Mar 16;17(6):1779-87
• All: 7 reference(s)