Krt6a/Krt6b^tm1Cou
Targeted Allele Detail

Summary

• Symbol: Krt6a/Krt6b^tm1Cou
• Name: keratin 6B; targeted mutation 1, Pierre A Coulombe
• MGI ID: MGI:2385808
• Synonyms: K6alpha/beta null
• Gene: Krt6b Location: Chr15:101584458-101588722 bp, - strand Genetic Position: Chr15, 56.97 cM
• Alliance: Krt6a/Krt6b^tm1Cou page

Growth retardation and tongue blisters in Krt6a/Krt6b^tm1Cou/Krt6a/Krt6b^tm1Cou mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64033
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Two closely linked genes were deleted using a single targeting event. The entire Krt2-6a gene, 13 kb of intergenic sequences, and the first half of Krt2-6b exon 1 were replaced with a neomycin resistance cassette via homologous recombination. RT-PCR and Northern blot analysis of cultured keratinocytes derived from homozygous mutant animals did not detect Krt2-6a or Krt2-6b transcripts, however a probe to the neo gene yielded positive results. Western blot analysis also failed to detect either Krt2-6a or Krt2-6b protein products. (J:64033)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Krt6b Mutation: 25 strains or lines available

References

• Original: J:64033 Wong P, et al., Introducing a null mutation in the mouse K6alpha and K6beta genes reveals their essential structural role in the oral mucosa. J Cell Biol. 2000 Aug 21;150(4):921-8
• All: 5 reference(s)