Fech^tm1Dab
Targeted Allele Detail

Summary

• Symbol: Fech^tm1Dab
• Name: ferrochelatase; targeted mutation 1, David A Brenner
• MGI ID: MGI:2384729
• Gene: Fech Location: Chr18:64589613-64622169 bp, - strand Genetic Position: Chr18, 37.43 cM
• Alliance: Fech^tm1Dab page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78345
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced exon 10 while leaving the slice donor and acceptor sites for exons 9 and 11 intact. RT-PCR and Western blot analysis showed the levels of both mutant transcript and protein to be approximately equivalent to those of normal transcript and protein in heterozygous mice. An assay based on chelation rate showed activity to be reduced by approximately 63% in heterozygous mutant mice. (J:52058, J:78345)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fech Mutation: 24 strains or lines available

References

• Original: J:78345 Magness ST, et al., An exon 10 deletion in the mouse ferrochelatase gene has a dominant-negative effect and causes mild protoporphyria. Blood. 2002 Aug 15;100(4):1470-7
• All: 2 reference(s)