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Targeted Allele Detail
Symbol: Fechtm1Dab
Name: ferrochelatase; targeted mutation 1, David A Brenner
MGI ID: MGI:2384729
Gene: Fech  Location: Chr18:64589613-64622169 bp, - strand  Genetic Position: Chr18, 37.43 cM
Alliance: Fechtm1Dab page
Germline Transmission:  Earliest citation of germline transmission: J:78345
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA neomycin selection cassette replaced exon 10 while leaving the slice donor and acceptor sites for exons 9 and 11 intact. RT-PCR and Western blot analysis showed the levels of both mutant transcript and protein to be approximately equivalent to those of normal transcript and protein in heterozygous mice. An assay based on chelation rate showed activity to be reduced by approximately 63% in heterozygous mutant mice. (J:52058, J:78345)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Fech Mutation:  15 strains or lines available
Original:  J:78345 Magness ST, et al., An exon 10 deletion in the mouse ferrochelatase gene has a dominant-negative effect and causes mild protoporphyria. Blood. 2002 Aug 15;100(4):1470-7
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory