Tg(KRT1-DSG3)1Dga
Transgene Detail

Summary

• Symbol: Tg(KRT1-DSG3)1Dga
• Name: transgene insertion 1, David R Garrod
• MGI ID: MGI:2384724
• Transgene: Tg(KRT1-DSG3)1Dga Location: unknown
• Alliance: Tg(KRT1-DSG3)1Dga page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(KRT1-DSG3)1Dga expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DSG3 (1830)

  Dsg3	(MGI:99499)

• Mutation details: A 3.3 kb full length human DSG3 cDNA under the control of the human keratin 1 promoter was used for the transgene. The promoter was used to target expression of the transgene to the upper (suprabasal) epidermal layer. Western blot analysis verified the presence of human protein in the epidermis of transgenic mice. Immunohistochemical analysis showed the presence of human protein in the suprabasal layers of transgenic epidermis. Southern blot determined that 13-15 copies of the transgene had integrated into the genome. (J:78071)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:78071 Merritt AJ, et al., Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation. Mol Cell Biol. 2002 Aug;22(16):5846-58
• All: 1 reference(s)