Nfatc1^tm2Glm
Targeted Allele Detail

Summary

• Symbol: Nfatc1^tm2Glm
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1; targeted mutation 2, Laurie H Glimcher
• MGI ID: MGI:2384395
• Gene: Nfatc1 Location: Chr18:80649420-80756286 bp, - strand Genetic Position: Chr18, 53.66 cM, cytoband E4
• Alliance: Nfatc1^tm2Glm page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:78376
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding 19 amino acids (635-653) of the Rel-homology domain were replaced with a hygromycin selection cassette. A frameshift mutation was introduced into the downstream sequence encoding amino acids 654 through 710. Western blot analysis showed the presence of truncated peptide in homozygous mutant mice. Electromobility shift assays showed a lack of DNA-binding activity. Generated in ES cells already carrying the Nfatc1^tm2Glm allele on the other chromosome. These mice were studied as chimeras made in blastocysts homozygous for an unspecified Rag2 knockout (J:78376)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfatc1 Mutation: 47 strains or lines available

Notes

Generated in ES cells already carrying the Nfatc1^tm2Glm allele on the other chromosome. These mice were studied as chimeras made in blastocysts homozygous for an unspecified Rag2 knockout.

References

• Original: J:78376 Ranger AM, et al., Delayed lymphoid repopulation with defects in IL-4-driven responses produced by inactivation of NF-ATc. Immunity. 1998 Jan;8(1):125-34
• All: 1 reference(s)