Insr^tm1Khn
Targeted Allele Detail

Summary

• Symbol: Insr^tm1Khn
• Name: insulin receptor; targeted mutation 1, Ronald Kahn
• MGI ID: MGI:2384332
• Synonyms: Insr^flox, IR^flox, IR(lox), IRlox, IR^Lox
• Gene: Insr Location: Chr8:3200922-3329649 bp, - strand Genetic Position: Chr8, 1.82 cM
• Alliance: Insr^tm1Khn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51266
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 4 was left flanked by single loxP sites after a downstream floxed neo cassette was removed via in vitro cre mediated recombination. Deletion of the resultant floxed fragment will result in a frameshift mutation that introduces a stop codon. Translation, if it were to occur, would putatively produce a truncated peptide consisting of 308 amino terminal residues and lacking the high affinity binding site, transmembrane domain, and kinase domain. (J:51266)

Disease models

Expression

In Mice Carrying this Mutation:	6 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	20 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Insr^tm1Khn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Insr Mutation: 95 strains or lines available

Notes

In conjunction with Tg(Mycha-cre)1Abel, this allele is knocked out specifically in cardiac muscle. The resulting mice are referred to as "Cardiac-specific insulin receptor knockout mice" or CIRKO.

In conjunction with Tg(Ckmm-cre)5Khn, this allele is disrupted specifically in skeletal muscle. The resulting mice are referred to as "Muscle-specific insulin receptor knockout mice" or MIRKO.

References

• Original: J:51266 Bruning JC, et al., A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance. Mol Cell. 1998 Nov;2(5):559-69
• All: 297 reference(s)