Cd3e^tm1Lov
Targeted Allele Detail

Summary

• Symbol: Cd3e^tm1Lov
• Name: CD3 antigen, epsilon polypeptide; targeted mutation 1, Paul E Love
• MGI ID: MGI:2384120
• Synonyms: CD3e^N
• Gene: Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3e^tm1Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72144
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A section of the genome encoding a 3' portion of exon 5, intron 5 and whole of exon 6, encoding the extracellular and transmembrane domains, was replaced by a floxed neo cassette. Northern blot analysis of total RNA obtained from thymocytes showed an absence of Cd3e transcript and reduced levels of Cd3d and Cd3g transcript in homozygous mutant mice. The downregulation of the delta and gamma polypeptides was believed to be due to the reverse transcriptional orientation of the neo transgene having an effect on the enhancer for Cd3d and Cd3g. To eliminate this effect, Cd3e^tm1.1Lov was generated by breeding mice possessing this allele with cre expressing mice. (J:72144)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd3e Mutation: 47 strains or lines available

References

• Original: J:72144 DeJarnette JB, et al., Specific requirement for CD3epsilon in T cell development. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14909-14
• All: 26 reference(s)