Kif3c^tm2Gsn
Targeted Allele Detail

Summary

• Symbol: Kif3c^tm2Gsn
• Name: kinesin family member 3C; targeted mutation 2, Lawrence S B Goldstein
• MGI ID: MGI:2183788
• Synonyms: Kif3c^typeII
• Gene: Kif3c Location: Chr12:3415132-3456494 bp, + strand Genetic Position: Chr12, 1.77 cM
• Alliance: Kif3c^tm2Gsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70607
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Two loxP sites were introduced to the intron and 5' untranslated region flanking exon 1, and a third loxP site was introduced with a PGK-TK/PGK-neo cassette directly downstream exon 1 via homologous recombination. The PGK-TK/PGK-neo cassette were removed by transient expression of cre recombinase in correctly targeted ES cells leaving the loxP sites flanking exon 1 intact. Gene expression in homozygous mutant animals appeared normal compared to wild-type animals as determined by Western blot analysis of brain lysates. (J:70607)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kif3c Mutation: 24 strains or lines available

References

• Original: J:70607 Yang Z, et al., Functional analysis of mouse kinesin motor kif3c. Mol Cell Biol. 2001 Aug;21(16):5306-11
• All: 1 reference(s)