Map2k7^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Map2k7^tm1Flv
• Name: mitogen-activated protein kinase kinase 7; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:2183768
• Synonyms: Mkk7^-
• Gene: Map2k7 Location: Chr8:4288740-4297897 bp, + strand Genetic Position: Chr8, 1.99 cM, cytoband A1.1
• Alliance: Map2k7^tm1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61895
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 3 exons were replaced by a floxed puromycin selection cassette, which was subsequently excised via transient transfection of ES cells with a cre expression vector. Western blot analysis of homozygous mutant ES cells showed an absence of encoded protein. (J:61895)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map2k7 Mutation: 19 strains or lines available

References

• Original: J:61895 Dong C, et al., JNK is required for effector T-cell function but not for T-cell activation. Nature. 2000 May 4;405(6782):91-4
• All: 3 reference(s)