Spag5^tm1Vdh
Targeted Allele Detail

Summary

• Symbol: Spag5^tm1Vdh
• Name: sperm associated antigen 5; targeted mutation 1, Frans A van der Hoorn
• MGI ID: MGI:2183294
• Synonyms: Spag5^-/-
• Gene: Spag5 Location: Chr11:78192412-78213283 bp, + strand Genetic Position: Chr11, 46.74 cM, cytoband B5
• Alliance: Spag5^tm1Vdh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75473
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.7 kb region of the gene containing exon 1 was replaced with a neomycin cassette via homologous recombination. Northern blot analysis of testes from homozygous mutant animals did not detect full length transcript; Western blot analysis of testicular germ cell failed to detect protein product. (J:75473)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spag5 Mutation: 40 strains or lines available

References

• Original: J:75473 Xue J, et al., Targeted disruption of the testicular SPAG5/deepest protein does not affect spermatogenesis or fertility. Mol Cell Biol. 2002 Apr;22(7):1993-7
• All: 1 reference(s)