Cldn1^tm1Sts
Targeted Allele Detail

Summary

• Symbol: Cldn1^tm1Sts
• Name: claudin 1; targeted mutation 1, Shoichiro Tsukida
• MGI ID: MGI:2183247
• Synonyms: claudin-1^-
• Gene: Cldn1 Location: Chr16:26175395-26190589 bp, - strand Genetic Position: Chr16, 18.0 cM
• Alliance: Cldn1^tm1Sts page

Cldn1^tm1Sts/Cldn1^tm1Sts mice show wrinkled skin and rapid postnatal lethality

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75447
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 1, which encodes the first transmembrane domain and first extracellular loop, with a PGK-neo cassette facing the opposite orientation via homologous recombination. Absence of gene expression in homozygous mutant mice was confirmed by Northern blot analysis and immunofluorescent microscopy of liver tissues. (J:75447)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cldn1 Mutation: 31 strains or lines available

References

• Original: J:75447 Furuse M, et al., Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. J Cell Biol. 2002 Mar 18;156(6):1099-111
• All: 4 reference(s)