Gfap^tm1Pkny
Targeted Allele Detail

Summary

• Symbol: Gfap^tm1Pkny
• Name: glial fibrillary acidic protein; targeted mutation 1, Milos Pekny
• MGI ID: MGI:2183221
• Synonyms: GFAP^-
• Gene: Gfap Location: Chr11:102778162-102791368 bp, - strand Genetic Position: Chr11, 66.48 cM, cytoband D
• Alliance: Gfap^tm1Pkny page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:25093
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette deleted 2.0kb of the upstream regulatory region as well as sequence in exon 1 encoding the first 71 amino acids. Northern blot analysis of total brain RNA showed a lack of transcript produced from this allele. Encoded protein was undetected in homozygous mutant mice via immunohistochemical staining of astrocytes. (J:25093, J:96523)

Tumor Data

List all tumor models in MMHCdb carrying Gfap^tm1Pkny

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gfap Mutation: 49 strains or lines available

References

• Original: J:25093 Pekny M, et al., Mice lacking glial fibrillary acidic protein display astrocytes devoid of intermediate filaments but develop and reproduce normally. EMBO J. 1995 Apr 18;14(8):1590-8
• All: 28 reference(s)