Gabra1^tm1.1Uru
Targeted Allele Detail

Summary

• Symbol: Gabra1^tm1.1Uru
• Name: gamma-aminobutyric acid type A receptor subunit alpha 1; targeted mutation 1.1, Uwe Rudolph
• MGI ID: MGI:2183155
• Synonyms: alpha1(H101R), Gabra1^{mutant allele 2}
• Gene: Gabra1 Location: Chr11:42021766-42073757 bp, - strand Genetic Position: Chr11, 24.97 cM
• Alliance: Gabra1^tm1.1Uru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75895
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: This allele was generated by breeding mice carrying Gabra1^tm1Uru with transgenic mice expressing cre recombinase via the EIIa promoter. The floxed neomycin cassette was excised from intron 4, while the point mutation in codon 101 that results in a histidine to arginine substitution remained intact. Normal levels and patterns of expression were determined via immunoblotting and immunohistochemical analysis of homozygous mutant mice. Receptors encoded by the targeted locus isolated from mutant mice showed ligand-binding profiles consistant with those of diazepam-insensitive receptors. (J:75895)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra1 Mutation: 47 strains or lines available

References

• Original: J:75895 Rudolph U, et al., Benzodiazepine actions mediated by specific gamma-aminobutyric acid(A) receptor subtypes. Nature. 1999 Oct 21;401(6755):796-800
• All: 9 reference(s)