Epx^tm1Nal
Targeted Allele Detail

Summary

• Symbol: Epx^tm1Nal
• Name: eosinophil peroxidase; targeted mutation 1, Nancy A Lee
• MGI ID: MGI:2182952
• Synonyms: EPO-
• Gene: Epx Location: Chr11:87754826-87766362 bp, - strand Genetic Position: Chr11, 52.25 cM
• Alliance: Epx^tm1Nal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70567
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 6-8 were replaced with a PGK-neo cassette via homologous recombination. These exons encode conserved histidine and arginine residues essential to catalytic activity, 6 half-cysteine residues involved in disulfide linkages, and 11 residues located near the heme prosthetic group. RT-PCR analysis revealed the absence of gene expression in various tissues from homozygous mutant animals. Loss of eosinophil peroxidase (EPO) activity in homozygous mutant mice was verified by 1) cytochemical staining of peritoneal cavity exudate cells recovered after sensitization/challenge of mice with a whole-protein extract from the helminth M. corti, and 2) an endpoint colorimetric assay detecting EPO in lysates of the peritoneal cavity exudates. In addition, loss of EPO activity was functionally demonstrated by assessing an EPO-specific oxidation product, bromotyrosine, in whole-lung homogenates from mutant mice using an OVA i.p. sensitization/aerosol challenge model of respiratory inflammation. (J:70567)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Epx Mutation: 34 strains or lines available

References

• Original: J:70567 Denzler KL, et al., Extensive eosinophil degranulation and peroxidase-mediated oxidation of airway proteins do not occur in a mouse ovalbumin-challenge model of pulmonary inflammation. J Immunol. 2001 Aug 1;167(3):1672-82
• All: 16 reference(s)