Per1^tm1Brd
Targeted Allele Detail

Summary

• Symbol: Per1^tm1Brd
• Name: period circadian clock 1; targeted mutation 1, Allan Bradley
• MGI ID: MGI:2182834
• Synonyms: mPer1-, mPer1^Brdm1, mPer1^m, Per1^-, Per1 KO
• Gene: Per1 Location: Chr11:68986043-69000786 bp, + strand Genetic Position: Chr11, 42.37 cM, cytoband B
• Alliance: Per1^tm1Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69769
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 5.3kb region, encompassing exons 4 through 18, was replaced by an Hprt minigene. The vector was designed such that the excision of the inserted cassette and potential subsequent splicing of exon 3 to exon 19 would introduce a frameshift mutation. Such a transcript was not found, however, a fusion transcript consisting of the first three exons of the endogenous gene aberrantly spliced to exons 3 through 9 of the Hprt transgene was identified by RT-PCR and sequence analysis. Because exons 1 through 3 encode a putatively non-functional region of the protein, the mutation was believed to be null. (J:69769)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Per1^tm1Brd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Per1 Mutation: 62 strains or lines available

References

• Original: J:69769 Zheng B, et al., Nonredundant roles of the mPer1 and mPer2 genes in the mammalian circadian clock. Cell. 2001 Jun 1;105(5):683-94
• All: 56 reference(s)