Podxl^tm1Kmn
Targeted Allele Detail

Summary

• Symbol: Podxl^tm1Kmn
• Name: podocalyxin-like; targeted mutation 1, Kelly M McNagny
• MGI ID: MGI:2182833
• Synonyms: podxl^-
• Gene: Podxl Location: Chr6:31496428-31540872 bp, - strand Genetic Position: Chr6, 12.57 cM
• Alliance: Podxl^tm1Kmn page

Edema in Podxl^tm1Kmn/Podxl^tm1Kmn mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70294
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of exons 5-8 were replaced with a neomycin resistance gene in the opposite orientation via homologous recombination. The gene targeting event deletes 55 amino acids in the extracellular/juxtamembrane region, the transmembrane region, and the entire cytoplasmic tail of the encoded protein. Exons 5, 7, and 8 encode highly conserved domains across species. Absence of gene expression was demonstrated by Northern blot analysis of E16 fetal lung using a 5' region of the gene as a probe. Lack of gene expression was further verified by cell surface immunofluorescence analysis of E15 fetal liver cells using a monoclonal antibody to the mouse protein. (J:70294)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Podxl Mutation: 18 strains or lines available

References

• Original: J:70294 Doyonnas R, et al., Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin. J Exp Med. 2001 Jul 2;194(1):13-27
• All: 6 reference(s)