Rhox9^tm1Dean
Targeted Allele Detail

Summary

• Symbol: Rhox9^tm1Dean
• Name: reproductive homeobox 9; targeted mutation 1, Jurrien Dean
• MGI ID: MGI:2182786
• Synonyms: Gpbox^tm
• Gene: Rhox9 Location: ChrX:36987974-36990647 bp, - strand Genetic Position: ChrX, 22.15 cM, cytoband A2
• Alliance: Rhox9^tm1Dean page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72863
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 2.2 kb of the gene, which contains the first 3 exons encoding the translation start site and most of the homeodomain, was replaced with a PGK-neo cassette via homologous recombination. RNase protection assay showed absence of gene expression in placentas and urogenital ridges of E12.5 hemizygous male and homozygous female mutant embryos. A small band intermittently observed may represent the alternative expression of 151 nt of exon 4. RT-PCR using primers to exons 2 and 4 further demonstrated the absence of gene transcription in the urogenital ridge of E12 mutant embryos. (J:72863)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhox9 Mutation: 1 strain or line available

References

• Original: J:72863 Takasaki N, et al., Normal Gonadal Development in Mice Lacking GPBOX, a Homeobox Protein Expressed in Germ Cells at the Onset of Sexual Dimorphism. Mol Cell Biol. 2001 Dec;21(23):8197-202
• All: 1 reference(s)