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Targeted Allele Detail
Symbol: Elanetm1Sds
Name: elastase, neutrophil expressed; targeted mutation 1, Steven D Shapiro
MGI ID: MGI:2182771
Synonyms: NE-
Gene: Elane  Location: Chr10:79722146-79724050 bp, + strand  Genetic Position: Chr10, 39.72 cM
Alliance: Elanetm1Sds page
Germline Transmission:  Earliest citation of germline transmission: J:47420
Parent Cell Line:  RW-4 (ES Cell)
Strain of Origin:  129X1/SvJ
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe insertion of a neomycin selection cassette replaced exon 2, which encodes the histidine residue of the catalytic triad. Northern blot analysis of total RNA obtained from bone marrow myeloid cells showed that transcript encoded by the targeted allele was absent. Reprobing of the blot showed that the expression of the closely linked proteinase 3 was unaffected by the targeting event. An absence of elastocytic activity derived from the targeted locus was determined via kappa-elastin zymography. (J:47420)
View phenotypes and curated references for all genotypes (concatenated display).
Tumor Data
List all tumor models in MMHCdb carrying Elanetm1Sds
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Elane Mutation:  18 strains or lines available
Original:  J:47420 Belaaouaj A, et al., Mice lacking neutrophil elastase reveal impaired host defense against gram negative bacterial sepsis. Nat Med. 1998 May;4(5):615-8
All:  66 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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MGI 6.22
The Jackson Laboratory