Eif2ak2^tm1Jcbe
Targeted Allele Detail

Summary

• Symbol: Eif2ak2^tm1Jcbe
• Name: eukaryotic translation initiation factor 2-alpha kinase 2; targeted mutation 1, John C Bell
• MGI ID: MGI:2182590
• Synonyms: C-PKR^-, Eif2ak2^em1Cya, PKR^-, Pkr^0/0
• Gene: Eif2ak2 Location: Chr17:79159993-79190002 bp, - strand Genetic Position: Chr17, 49.56 cM, cytoband E2
• Alliance: Eif2ak2^tm1Jcbe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53258
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 12, encoding subdomains 5 and 6 of the catalytic domain, was replaced with a neomycin selection cassette. Northern blot analysis of total RNA isolated from ES cells identified the production of a novel transcript containing the neo transgene. RT-PCR analysis confirmed that transcription was derived from the endogenous promoter with read-through into the transgene. Western blot analysis failed to detect encoded protein in cell lysates from homozygous mutant mice. The functional ablation of the protein was determined via an immune complex kinase autophosphorylation assays. (J:53258)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Eif2ak2 Mutation: 37 strains or lines available

References

• Original: J:53258 Abraham N, et al., Characterization of transgenic mice with targeted disruption of the catalytic domain of the double-stranded RNA-dependent protein kinase, PKR. J Biol Chem. 1999 Feb 26;274(9):5953-62
• All: 24 reference(s)