Ncan^tm3Ref
Targeted Allele Detail

Summary

• Symbol: Ncan^tm3Ref
• Name: neurocan; targeted mutation 3, Reinhard Fassler
• MGI ID: MGI:2182151
• Synonyms: NCCre
• Gene: Ncan Location: Chr8:70545735-70573494 bp, - strand Genetic Position: Chr8, 34.15 cM
• Alliance: Ncan^tm3Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70858
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A region encompassing the TATA box, the transcription initiation site, and exons 1 and 2 was replaced with a floxed neo cassette that was subsequently excised via the in vitro expression of cre recombinase. Northern and Western blot analyses showed an absence of transcript and protein in homozygous mutant mice. (J:70858)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncan Mutation: 46 strains or lines available

References

• Original: J:70858 Zhou XH, et al., Neurocan is dispensable for brain development. Mol Cell Biol. 2001 Sep;21(17):5970-8
• All: 6 reference(s)