Cst3^tm1Karl
Targeted Allele Detail

Summary

• Symbol: Cst3^tm1Karl
• Name: cystatin C; targeted mutation 1, S Karlsson
• MGI ID: MGI:2182140
• Synonyms: Cstc^-, CstC^-, Cys68, CysC^-
• Gene: Cst3 Location: Chr2:148713642-148717432 bp, - strand Genetic Position: Chr2, 73.6 cM
• Alliance: Cst3^tm1Karl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62407
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An attempted knockin resulted in the generation of a null mouse. The majority of exon 1 was replaced by a human Cst3 cDNA fragment containing a missense mutation that substitutes a glutamine for a leucine at codon 68. In addition, a neomycin selection cassette was inserted at the 3' end of exon 1 and replaced two thirds of intron 1. Northern blot analysis using probes for both the human and mouse transcript showed an absence of both transcripts in lung, heart, testis, kidney and brain tissues obtained from homozygous mutant mice. ELIZA using human- and mouse-specific antibodies failed to detect either protein in homozygous mutant animals. (J:62407)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cst3 Mutation: 15 strains or lines available

References

• Original: J:62407 Huh CG, et al., Decreased metastatic spread in mice homozygous for a null allele of the cystatin C protease inhibitor gene. Mol Pathol. 1999 Dec;52(6):332-40
• All: 18 reference(s)