Kcnj5^tm1Clph
Targeted Allele Detail

Summary

• Symbol: Kcnj5^tm1Clph
• Name: potassium inwardly-rectifying channel, subfamily J, member 5; targeted mutation 1, David E Clapham
• MGI ID: MGI:2181760
• Synonyms: GIRK4 (-)
• Gene: Kcnj5 Location: Chr9:32226002-32255640 bp, - strand Genetic Position: Chr9, 17.65 cM
• Alliance: Kcnj5^tm1Clph page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45533
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced exon 1, which encodes the amino and carboxyl termini, both transmembrane domains, and the putative pore-lining sequence. Northern blot analysis of total atrial RNA showed an absence of normal transcript in homozygous mutant mice. mRNA levels of other K⁺ inwardly-rectifying channels, were shown to be unaffected by the targeting event by rehybridization of the Northern blot using Kcnj3-, Kcnj6- and Kcnj9-specific probes. Western blot analysis of protein extracts derived from atrial cell membranes showed that the targeted Kcnj5 protein product was absent, and levels of both glycosylated and unglycosylated forms of Kcnj3 were significantly reduced in homozygous mutant mice. (J:45533)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnj5 Mutation: 29 strains or lines available

References

• Original: J:45533 Wickman K, et al., Abnormal heart rate regulation in GIRK4 knockout mice. Neuron. 1998 Jan;20(1):103-14
• All: 21 reference(s)