Crk^{Gt(pU-Hachi)8104Imeg}
Gene trapped Allele Detail

Summary

• Symbol: Crk^{Gt(pU-Hachi)8104Imeg}
• Name: v-crk avian sarcoma virus CT10 oncogene homolog; gene trap 8104, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:2181183
• Gene: Crk Location: Chr11:75570085-75597734 bp, + strand Genetic Position: Chr11, 45.92 cM, cytoband A-D
• Alliance: Crk^{Gt(pU-Hachi)8104Imeg} page

Mutation origin

• Mutant Cell Line: AYU8104
• Germline Transmission: Earliest citation of germline transmission: J:59071
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The integration of a gene trap vector into the coding sequence of the endogenous allele resulted in an isoform specific disruption. Sequence analysis revealed that the insertion prevented the translation of the carboxy terminal SH3 domain, thereby affecting the isoform containing both SH3 domains and not the isoform that contains only the amino terminal SH3 domain. Western blot analysis of homozygous mutant mice showed the presence of two mutant isoforms structurally similar to the wild-type splice variant containing single the single SH3 domains but lacking a tyrosine at position 221 and having additional amino acids encoded from the gene trap vector splice acceptor sequence on the carboxyl terminus. (J:59071)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crk Mutation: 22 strains or lines available

References

• Original: J:59071 Imaizumi T, et al., Mutant mice lacking Crk-II caused by the gene trap insertional mutagenesis: Crk-II is not essential for embryonic development. Biochem Biophys Res Commun. 1999 Dec 20;266(2):569-74
• All: 2 reference(s)