Cd9^tm1Kudo
Targeted Allele Detail

Summary

• Symbol: Cd9^tm1Kudo
• Name: CD9 antigen; targeted mutation 1, Akira Kudo
• MGI ID: MGI:2180798
• Synonyms: Cd9^-
• Gene: Cd9 Location: Chr6:125437229-125471723 bp, - strand Genetic Position: Chr6, 59.32 cM
• Alliance: Cd9^tm1Kudo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60753
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2, which encodes the first transmembrane domain, was replaced with a neomycin resistance gene. Sequenc analysis of a transcript present in mutant mice showed that exon 1 had spliced to exon 3 causing a frameshift mutation that introduced a stop codon in exon 3. While a cytoplasmic domain may have been produced, flow cytometric analysis of bone marrow macrophages showed no cell surface protein expression. (J:60753)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd9 Mutation: 101 strains or lines available

References

• Original: J:60753 Kaji K, et al., The gamete fusion process is defective in eggs of Cd9-deficient mice. Nat Genet. 2000 Mar;24(3):279-82
• All: 1 reference(s)