Aldh3a1^tm1Jpi
Targeted Allele Detail

Summary

• Symbol: Aldh3a1^tm1Jpi
• Name: aldehyde dehydrogenase family 3, subfamily A1; targeted mutation 1, Joram Piatigorsky
• MGI ID: MGI:2180794
• Synonyms: ALDH3a1-
• Gene: Aldh3a1 Location: Chr11:61099336-61109244 bp, + strand Genetic Position: Chr11, 37.96 cM
• Alliance: Aldh3a1^tm1Jpi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73968
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced the 1.2 kb region spanning from within exons 2 to 3 and placed an in-frame translation termination codon immediately after the initiator methionine codon in exon 2, and a putative eukaryotic transcriptional terminator (E. coli tryptophan attenuator) between the inserted stop codon and the neomycin gene. Immunoblot analysis using a rabbit anti-rat antibody did not detect protein in corneal epithelial extracts from homozygous mutant mice. Zymogram analysis of corneal epithelial extracts from homozygous mutant mice did not detect protein activity. (J:73968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aldh3a1 Mutation: 25 strains or lines available

References

• Original: J:73968 Nees DW, et al., Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice. Mol Cell Biol. 2002 Feb;22(3):849-55
• All: 5 reference(s)