Cd59a^tm1Bpm
Targeted Allele Detail

Summary

• Symbol: Cd59a^tm1Bpm
• Name: CD59a antigen; targeted mutation 1, B Paul Morgan
• MGI ID: MGI:2180771
• Synonyms: CD59^-, Cd59a-, mCd59a^-
• Gene: Cd59a Location: Chr2:103926146-103945699 bp, + strand Genetic Position: Chr2, 54.53 cM, cytoband E2-E4
• Alliance: Cd59a^tm1Bpm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70419
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette was inserted to replace exon 3, which encodes the majority of the protein. FACS analysis showed an absence of protein expression on the surface of erythrocytes, leukocytes, and platelets isolated from homozygous mutant mice. The lack of protein expression was confirmed by immunhistochemical analysis in a variety of tissues. (J:70419)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd59a Mutation: 18 strains or lines available

References

• Original: J:70419 Holt DS, et al., Targeted deletion of the CD59 gene causes spontaneous intravascular hemolysis and hemoglobinuria. Blood. 2001 Jul 15;98(2):442-9
• All: 26 reference(s)