Cd22^tm1Tft
Targeted Allele Detail

Summary

• Symbol: Cd22^tm1Tft
• Name: CD22 antigen; targeted mutation 1, Thomas F Tedder
• MGI ID: MGI:2180700
• Synonyms: CD22-
• Gene: Cd22 Location: Chr7:30564829-30579767 bp, - strand Genetic Position: Chr7, 19.26 cM
• Alliance: Cd22^tm1Tft page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38051
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette replaced a portion of the 5' untranslated region, the leader sequence, and a portion of the exon encoding the immunoglobulin (Ig) 1 domain. A stop codon was introduced into the 5' end of the exon encoding the Ig 1 domain. Immunohistochemical staining of frozen spleen sections, using various Ig-specific antibodies, showed a lack of secreted, cell surface, and cytoplasmic protein. The absence of sell surface expression was further confirmed by immunofluorescence staining of lymphocytes. (J:38051)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd22 Mutation: 59 strains or lines available

References

• Original: J:38051 Sato S, et al., CD22 is both a positive and negative regulator of B lymphocyte antigen receptor signal transduction: altered signaling in CD22-deficient mice. Immunity. 1996 Dec;5(6):551-62
• All: 19 reference(s)