Ckmt1^tm2Bew
Targeted Allele Detail

Summary

• Symbol: Ckmt1^tm2Bew
• Name: creatine kinase, mitochondrial 1, ubiquitous; targeted mutation 2, Be Wieringa
• MGI ID: MGI:2180567
• Synonyms: UbCKmit-deficient
• Gene: Ckmt1 Location: Chr2:121188257-121194218 bp, + strand Genetic Position: Chr2, 60.37 cM, cytoband F1-F3
• Alliance: Ckmt1^tm2Bew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:27221
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.6 kb segment of the gene containing exons 7 and 8 was replaced with a neo resistance cassette via homologous recombination resulting in elimination of a catalytically significant cysteine. Unexpected banding patterns were observed in Southern blot analysis of homozygous mutant animals and may be the result of integration of an aberrantly ligated targeting vector. Homozygous mutant animals lacked enzyme activity as demonstrated by enzyme staining assays of brain extracts. (J:27221)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ckmt1 Mutation: 31 strains or lines available

References

• Original: J:27221 Steeghs K, et al., Mice deficient in ubiquitous mitochondrial creatine kinase are viable and fertile. Biochim Biophys Acta. 1995 Jun 30;1230(3):130-8
• All: 9 reference(s)