Capn1^tm1Ahc
Targeted Allele Detail

Summary

• Symbol: Capn1^tm1Ahc
• Name: calpain 1; targeted mutation 1, Athar H Chishti
• MGI ID: MGI:2180538
• Synonyms: Capn^-
• Gene: Capn1 Location: Chr19:6038573-6065855 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Capn1^tm1Ahc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67798
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette replaced the portion of exon 4 encoding amino acids 153 through 160, part of an essential catalytic domain. Expression analysis of homozygous mutant mice showed an absence of normal transcript and additionally that alternate trancripts were not produced by cryptic splicing. Western analysis revealed a lack of protein in homozygous mutants, while casein zymography did not detect calpain activity in platelets. (J:67798)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Capn1 Mutation: 33 strains or lines available

References

• Original: J:67798 Azam M, et al., Disruption of the mouse mu-calpain gene reveals an essential role in platelet function. Mol Cell Biol. 2001 Mar;21(6):2213-20
• All: 20 reference(s)