Adcyap1r1^tm1Bbt
Targeted Allele Detail

Summary

• Symbol: Adcyap1r1^tm1Bbt
• Name: adenylate cyclase activating polypeptide 1 receptor 1; targeted mutation 1, Philippe Brabet
• MGI ID: MGI:2180097
• Synonyms: PAC1-
• Gene: Adcyap1r1 Location: Chr6:55428963-55478436 bp, + strand Genetic Position: Chr6, 27.38 cM, cytoband B3
• Alliance: Adcyap1r1^tm1Bbt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62115
• Parent Cell Line: H1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a 2.3-kb fragment containing exons 8 through 11. The neo gene was situated in the reverse transcriptional orientation and was excised during the splicing of the transcript, resulting in a frameshift that introduces a stop codon in exon 12. A truncated transcript was observed by RT-PCR analysis. A 90% decrease in binding of a radio-labeled ligand in homozygous mutants was observed versus wild-type, as well as an absence of cAMP production stimulation. (J:62115)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adcyap1r1 Mutation: 34 strains or lines available

References

• Original: J:62115 Jamen F, et al., PAC1 receptor-deficient mice display impaired insulinotropic response to glucose and reduced glucose tolerance. J Clin Invest. 2000 May;105(9):1307-15
• All: 17 reference(s)