Mak^tm1Yshk
Targeted Allele Detail

Summary

• Symbol: Mak^tm1Yshk
• Name: male germ cell-associated kinase; targeted mutation 1, Yoichi Shinkai
• MGI ID: MGI:2179972
• Synonyms: Mak-
• Gene: Mak Location: Chr13:41178484-41233182 bp, - strand Genetic Position: Chr13, 20.25 cM
• Alliance: Mak^tm1Yshk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76245
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5-8 were replaced with a neo cassette via homologous recombination resulting in deletion of 45% of the protein product including a kinase domain and PQ-rich domain. Western blot of testicular samples from homozygous mutant animals verified the absence of protein and enzyme assays were negative for kinase activity. (J:76245)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mak Mutation: 34 strains or lines available

References

• Original: J:76245 Shinkai Y, et al., A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation. Mol Cell Biol. 2002 May;22(10):3276-80
• All: 3 reference(s)