Smad2^tm1Rak
Targeted Allele Detail

Summary

• Symbol: Smad2^tm1Rak
• Name: SMAD family member 2; targeted mutation 1, Raju Kucherlapati
• MGI ID: MGI:2179521
• Synonyms: Smad2^dex2
• Gene: Smad2 Location: Chr18:76374651-76444034 bp, + strand Genetic Position: Chr18, 51.42 cM
• Alliance: Smad2^tm1Rak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73507
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 and a 1.5 kb region preceding exon 2 were replaced with a PGK-neo cassette via homologous recombination resulting in deletion of the start codon. Western blot of protein extracts from homozygous mutant animals confirmed the absence of protein product. (J:73507)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad2 Mutation: 50 strains or lines available

References

• Original: J:73507 Heyer J, et al., Postgastrulation Smad2-deficient embryos show defects in embryo turning and anterior morphogenesis. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12595-600
• All: 2 reference(s)