Gabra6^tm1Wwis
Targeted Allele Detail

Summary

• Symbol: Gabra6^tm1Wwis
• Name: gamma-aminobutyric acid type A receptor subunit alpha 6; targeted mutation 1, W Wisden
• MGI ID: MGI:2179278
• Synonyms: alpha6-, deltaalpha6lacZ
• Gene: Gabra6 Location: Chr11:42197264-42211899 bp, - strand Genetic Position: Chr11, 25.03 cM
• Alliance: Gabra6^tm1Wwis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75896
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 8 of this gene was replaced with an IRES-lacZneo cassette. The targeting vector introduced stop codons in all 3 reading frames and results in protein truncation after the TM2 region. Beta-gal staining was positive in the cerebellar granule layer of homozygous mutant animals. Absence of gene expression was confirmed by immunocytochemistry of the cerebellar granule layer and Western blot of cerebellar protein extracts. (J:75896)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabra6 Mutation: 38 strains or lines available

References

• Original: J:75896 Jones A, et al., Ligand-gated ion channel subunit partnerships: GABAA receptor alpha6 subunit gene inactivation inhibits delta subunit expression. J Neurosci. 1997 Feb 15;17(4):1350-62
• All: 8 reference(s)