Rad51^tm1Tkm
Targeted Allele Detail

Summary

• Symbol: Rad51^tm1Tkm
• Name: RAD51 recombinase; targeted mutation 1, Takashi Morita
• MGI ID: MGI:2179218
• Synonyms: Rad51-
• Gene: Rad51 Location: Chr2:118943295-118966554 bp, + strand Genetic Position: Chr2, 59.92 cM, cytoband F1
• Alliance: Rad51^tm1Tkm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33832
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 54 nucleotides of exon 5 and 187 nucleotides of intron 5 were replaced with a neo-derived cassette via homologous recombination resulting in the deletion of the ATP-binding motif of domain II responsible for DNA repair and recombination. Homozygous mutant embryos could only be detected before the 4- to 8- cell stage by PCR analysis. (J:33832)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad51 Mutation: 32 strains or lines available

References

• Original: J:33832 Tsuzuki T, et al., Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6236-40
• All: 1 reference(s)