Mapk14^tm1Nbr
Targeted Allele Detail

Summary

• Symbol: Mapk14^tm1Nbr
• Name: mitogen-activated protein kinase 14; targeted mutation 1, Angel R Nebreda
• MGI ID: MGI:2177831
• Synonyms: p38alpha -
• Gene: Mapk14 Location: Chr17:28910303-28967380 bp, + strand Genetic Position: Chr17, 14.85 cM
• Alliance: Mapk14^tm1Nbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63877
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An internal 2 kb genomic fragment was replaced with a lacZ gene and a PGK-neomycin cassette. The deleted region contains two exons encoding amino acids 40-103, which includes most of the amino-terminal lobe of the catalytic domain including the glycine-rich ATP binding loop. Western blot analysis of lysates prepared from E10.5 embryos confirmed that no detectable protein was expressed from this allele. (J:63877)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapk14 Mutation: 41 strains or lines available

References

• Original: J:63877 Adams RH, et al., Essential role of p38alpha MAP kinase in placental but not embryonic cardiovascular development. Mol Cell. 2000 Jul;6(1):109-16
• All: 7 reference(s)