Gc^tm1Nec
Targeted Allele Detail

Summary

• Symbol: Gc^tm1Nec
• Name: vitamin D binding protein; targeted mutation 1, Nancy E Cooke
• MGI ID: MGI:2176841
• Synonyms: DBP-
• Gene: Gc Location: Chr5:89565381-89605757 bp, - strand Genetic Position: Chr5, 44.32 cM
• Alliance: Gc^tm1Nec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52225
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a PGK-neomycin resistance cassette into exon 5 disrupted the gene. Northern analysis using a rat cDNA probe did not detect wild-type mRNA in liver from homozygous mutant mice. Neither western blot analysis nor simple radial immunodiffusion assay detected protein in sera from homozygous mutant mice. (J:52225)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gc Mutation: 25 strains or lines available

References

• Original: J:52225 Safadi FF, et al., Osteopathy and resistance to vitamin D toxicity in mice null for vitamin D binding protein. J Clin Invest. 1999 Jan;103(2):239-51
• All: 7 reference(s)